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primary antibodies against dgka  (Proteintech)


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    Structured Review

    Proteintech primary antibodies against dgka
    Primers used in this study.
    Primary Antibodies Against Dgka, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+against+dgka/DGKA+Antibody/pmc10151139-92-3-7
    Average 93 stars, based on 17 article reviews
    primary antibodies against dgka - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Targeting diacylglycerol kinase α impairs lung tumorigenesis by inhibiting cyclin D3"

    Article Title: Targeting diacylglycerol kinase α impairs lung tumorigenesis by inhibiting cyclin D3

    Journal: Thoracic Cancer

    doi: 10.1111/1759-7714.14851

    Primers used in this study.
    Figure Legend Snippet: Primers used in this study.

    Techniques Used: Sequencing

    DGKA silencing downregulates the expression of CCND3. (a) Volcano plot showing differentially expressed genes in DGKA‐depleted vs. scramble siRNA‐transfected A549 cells. (b) Venn diagram showing the number of genes in each indicated set. (c) Enrichment of differentially expressed genes in the pathways is illustrated in a Kyoto Encyclopedia of Genes and Genomes enrichment bubble chart. Y‐axis represents the pathways, and the X‐axis represents rich ratio. (d) Clustering heatmap of eight deregulated genes affected by loss of DGKA. (e) A549 and H1299 cells were transfected with the indicated siRNAs. After 48 h, the cells were harvested, and the levels of indicated mRNAs were measured by real‐time qPCR. *** p < 0.001 ( n = 3). (f) Knockdown of DGKA led to decreased protein levels of CCND3 in A549 and AH1299 cells. (g) Representative images of IHC staining for DGKA and CCND3 in xenograft tumors in the ritanserin‐administered group and vehicle group and statistical histograms of the IHC score. * p < 0.05 ( n = 5). Scale bar: 10 μm.
    Figure Legend Snippet: DGKA silencing downregulates the expression of CCND3. (a) Volcano plot showing differentially expressed genes in DGKA‐depleted vs. scramble siRNA‐transfected A549 cells. (b) Venn diagram showing the number of genes in each indicated set. (c) Enrichment of differentially expressed genes in the pathways is illustrated in a Kyoto Encyclopedia of Genes and Genomes enrichment bubble chart. Y‐axis represents the pathways, and the X‐axis represents rich ratio. (d) Clustering heatmap of eight deregulated genes affected by loss of DGKA. (e) A549 and H1299 cells were transfected with the indicated siRNAs. After 48 h, the cells were harvested, and the levels of indicated mRNAs were measured by real‐time qPCR. *** p < 0.001 ( n = 3). (f) Knockdown of DGKA led to decreased protein levels of CCND3 in A549 and AH1299 cells. (g) Representative images of IHC staining for DGKA and CCND3 in xenograft tumors in the ritanserin‐administered group and vehicle group and statistical histograms of the IHC score. * p < 0.05 ( n = 5). Scale bar: 10 μm.

    Techniques Used: Expressing, Transfection, Knockdown, Immunohistochemistry

    The inhibitory effect of DGKA silencing on the proliferation of lung cancer cells is partly dependent on CCND3. (a) A549 and H1299 cells were harvested and analyzed by western blotting after transfected with the siRNAs targeted DGKA and Flag‐CCND3. (b) A549 and H1299 cells transfected with the targeting siRNAs and Flag‐CCND3 were cultured in a 6‐well plate in triplicate and grown for 7 to 14 days. *** p < 0.001 ( n = 3). (c) EdU assay was used to detect the proliferation of A549 and H1299 cells transfected with the indicated siRNAs and Flag‐CCND3. *** p < 0.001 ( n = 3). Scale bar: 50 μm.
    Figure Legend Snippet: The inhibitory effect of DGKA silencing on the proliferation of lung cancer cells is partly dependent on CCND3. (a) A549 and H1299 cells were harvested and analyzed by western blotting after transfected with the siRNAs targeted DGKA and Flag‐CCND3. (b) A549 and H1299 cells transfected with the targeting siRNAs and Flag‐CCND3 were cultured in a 6‐well plate in triplicate and grown for 7 to 14 days. *** p < 0.001 ( n = 3). (c) EdU assay was used to detect the proliferation of A549 and H1299 cells transfected with the indicated siRNAs and Flag‐CCND3. *** p < 0.001 ( n = 3). Scale bar: 50 μm.

    Techniques Used: Western Blot, Transfection, Cell Culture, EdU Assay

    Related Articles

    Cell Culture:

    Article Title: Targeting diacylglycerol kinase α impairs lung tumorigenesis by inhibiting cyclin D3
    Article Snippet: .. Next, the applicable primary antibodies against DGKA (Proteintech, 11 547‐1‐AP, 1:6000), CCND3 (Proteintech, 26 755‐1‐AP, 1:1000), MAD2L1 (Proteintech, 10 337‐1‐AP, 1:1000), PKMYT1 (Proteintech, 67 806‐1‐Ig, 1:5000), CCND1 (Proteintech, 60 186‐1‐Ig, 1:10000), ESPL1 (ABclonal, A15366, 1:1000), E2F2 (Affinity, AF4100, 1:1000), and glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) (Servicebio, GB11002, 1:1000) were cultured overnight with PVDF membranes at 4°C. ..

    Western Blot:

    Article Title: Epigenetic regulation of diacylglycerol kinase alpha promotes radiation-induced fibrosis
    Article Snippet: Total protein was isolated by lysing cells in RIPA lysis buffer (50 mM Tris-HCl pH 8.0, 150 mM NaCl 1.0% (v/v) nonidet-P40, 0.5% m/v sodium deoxycholate, 0.1% m/v sodium dodecyl sulfate) supplemented with protease inhibitor (Roche). .. Western blot analysis was run with NuPAGE SDS-PAGE gel system (Life Technologies) and primary antibodies against DGKA (11547-1-AP, Proteintech), beta actin (ACTB; sc-47778 HRP, Santa Cruz Biotechnology), PRKCA (2056P, Cell Signaling Technology), collagen 1 (EPR7785, Abcam) and appropriate secondary antibodies (Santa Cruz Biotechnology). .. Detection was carried out using ECL reagent (Perkin-Elmer) and uncropped western Blot images are provided in .

    SDS Page:

    Article Title: Epigenetic regulation of diacylglycerol kinase alpha promotes radiation-induced fibrosis
    Article Snippet: Total protein was isolated by lysing cells in RIPA lysis buffer (50 mM Tris-HCl pH 8.0, 150 mM NaCl 1.0% (v/v) nonidet-P40, 0.5% m/v sodium deoxycholate, 0.1% m/v sodium dodecyl sulfate) supplemented with protease inhibitor (Roche). .. Western blot analysis was run with NuPAGE SDS-PAGE gel system (Life Technologies) and primary antibodies against DGKA (11547-1-AP, Proteintech), beta actin (ACTB; sc-47778 HRP, Santa Cruz Biotechnology), PRKCA (2056P, Cell Signaling Technology), collagen 1 (EPR7785, Abcam) and appropriate secondary antibodies (Santa Cruz Biotechnology). .. Detection was carried out using ECL reagent (Perkin-Elmer) and uncropped western Blot images are provided in .



    Similar Products

    primary antibodies against dgka  (Proteintech)
    93
    Proteintech primary antibodies against dgka
    Primers used in this study.
    Primary Antibodies Against Dgka, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+against+dgka/DGKA+Antibody/pmc10151139-92-3-7
    Average 93 stars, based on 1 article reviews
    primary antibodies against dgka - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Primers used in this study.

    Journal: Thoracic Cancer

    Article Title: Targeting diacylglycerol kinase α impairs lung tumorigenesis by inhibiting cyclin D3

    doi: 10.1111/1759-7714.14851

    Figure Lengend Snippet: Primers used in this study.

    Article Snippet: Next, the applicable primary antibodies against DGKA (Proteintech, 11 547‐1‐AP, 1:6000), CCND3 (Proteintech, 26 755‐1‐AP, 1:1000), MAD2L1 (Proteintech, 10 337‐1‐AP, 1:1000), PKMYT1 (Proteintech, 67 806‐1‐Ig, 1:5000), CCND1 (Proteintech, 60 186‐1‐Ig, 1:10000), ESPL1 (ABclonal, A15366, 1:1000), E2F2 (Affinity, AF4100, 1:1000), and glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) (Servicebio, GB11002, 1:1000) were cultured overnight with PVDF membranes at 4°C.

    Techniques: Sequencing

    DGKA silencing downregulates the expression of CCND3. (a) Volcano plot showing differentially expressed genes in DGKA‐depleted vs. scramble siRNA‐transfected A549 cells. (b) Venn diagram showing the number of genes in each indicated set. (c) Enrichment of differentially expressed genes in the pathways is illustrated in a Kyoto Encyclopedia of Genes and Genomes enrichment bubble chart. Y‐axis represents the pathways, and the X‐axis represents rich ratio. (d) Clustering heatmap of eight deregulated genes affected by loss of DGKA. (e) A549 and H1299 cells were transfected with the indicated siRNAs. After 48 h, the cells were harvested, and the levels of indicated mRNAs were measured by real‐time qPCR. *** p < 0.001 ( n = 3). (f) Knockdown of DGKA led to decreased protein levels of CCND3 in A549 and AH1299 cells. (g) Representative images of IHC staining for DGKA and CCND3 in xenograft tumors in the ritanserin‐administered group and vehicle group and statistical histograms of the IHC score. * p < 0.05 ( n = 5). Scale bar: 10 μm.

    Journal: Thoracic Cancer

    Article Title: Targeting diacylglycerol kinase α impairs lung tumorigenesis by inhibiting cyclin D3

    doi: 10.1111/1759-7714.14851

    Figure Lengend Snippet: DGKA silencing downregulates the expression of CCND3. (a) Volcano plot showing differentially expressed genes in DGKA‐depleted vs. scramble siRNA‐transfected A549 cells. (b) Venn diagram showing the number of genes in each indicated set. (c) Enrichment of differentially expressed genes in the pathways is illustrated in a Kyoto Encyclopedia of Genes and Genomes enrichment bubble chart. Y‐axis represents the pathways, and the X‐axis represents rich ratio. (d) Clustering heatmap of eight deregulated genes affected by loss of DGKA. (e) A549 and H1299 cells were transfected with the indicated siRNAs. After 48 h, the cells were harvested, and the levels of indicated mRNAs were measured by real‐time qPCR. *** p < 0.001 ( n = 3). (f) Knockdown of DGKA led to decreased protein levels of CCND3 in A549 and AH1299 cells. (g) Representative images of IHC staining for DGKA and CCND3 in xenograft tumors in the ritanserin‐administered group and vehicle group and statistical histograms of the IHC score. * p < 0.05 ( n = 5). Scale bar: 10 μm.

    Article Snippet: Next, the applicable primary antibodies against DGKA (Proteintech, 11 547‐1‐AP, 1:6000), CCND3 (Proteintech, 26 755‐1‐AP, 1:1000), MAD2L1 (Proteintech, 10 337‐1‐AP, 1:1000), PKMYT1 (Proteintech, 67 806‐1‐Ig, 1:5000), CCND1 (Proteintech, 60 186‐1‐Ig, 1:10000), ESPL1 (ABclonal, A15366, 1:1000), E2F2 (Affinity, AF4100, 1:1000), and glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) (Servicebio, GB11002, 1:1000) were cultured overnight with PVDF membranes at 4°C.

    Techniques: Expressing, Transfection, Knockdown, Immunohistochemistry

    The inhibitory effect of DGKA silencing on the proliferation of lung cancer cells is partly dependent on CCND3. (a) A549 and H1299 cells were harvested and analyzed by western blotting after transfected with the siRNAs targeted DGKA and Flag‐CCND3. (b) A549 and H1299 cells transfected with the targeting siRNAs and Flag‐CCND3 were cultured in a 6‐well plate in triplicate and grown for 7 to 14 days. *** p < 0.001 ( n = 3). (c) EdU assay was used to detect the proliferation of A549 and H1299 cells transfected with the indicated siRNAs and Flag‐CCND3. *** p < 0.001 ( n = 3). Scale bar: 50 μm.

    Journal: Thoracic Cancer

    Article Title: Targeting diacylglycerol kinase α impairs lung tumorigenesis by inhibiting cyclin D3

    doi: 10.1111/1759-7714.14851

    Figure Lengend Snippet: The inhibitory effect of DGKA silencing on the proliferation of lung cancer cells is partly dependent on CCND3. (a) A549 and H1299 cells were harvested and analyzed by western blotting after transfected with the siRNAs targeted DGKA and Flag‐CCND3. (b) A549 and H1299 cells transfected with the targeting siRNAs and Flag‐CCND3 were cultured in a 6‐well plate in triplicate and grown for 7 to 14 days. *** p < 0.001 ( n = 3). (c) EdU assay was used to detect the proliferation of A549 and H1299 cells transfected with the indicated siRNAs and Flag‐CCND3. *** p < 0.001 ( n = 3). Scale bar: 50 μm.

    Article Snippet: Next, the applicable primary antibodies against DGKA (Proteintech, 11 547‐1‐AP, 1:6000), CCND3 (Proteintech, 26 755‐1‐AP, 1:1000), MAD2L1 (Proteintech, 10 337‐1‐AP, 1:1000), PKMYT1 (Proteintech, 67 806‐1‐Ig, 1:5000), CCND1 (Proteintech, 60 186‐1‐Ig, 1:10000), ESPL1 (ABclonal, A15366, 1:1000), E2F2 (Affinity, AF4100, 1:1000), and glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) (Servicebio, GB11002, 1:1000) were cultured overnight with PVDF membranes at 4°C.

    Techniques: Western Blot, Transfection, Cell Culture, EdU Assay